Complete sequence analysis of mitochondrial DNA and telomere length in aplastic anemia

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Complete sequence analysis of mitochondrial DNA of aplastic anemia patients.

This study was primarily undertaken to test the hypothesis that mitochondrial DNA (mtDNA) mutations may be associated with aplastic anemia (AA). We analyzed mtDNA sequences from 15 patients with AA. The samples were obtained from bone marrow, and patients' oral epithelial cells were collected for normal tissue comparison. Total DNA was amplified by PCR after extraction, and these segments ...

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Telomere length in leukocyte subpopulations of patients with aplastic anemia.

In most human cells, the average length of telomere repeats at the ends of chromosomes provides indirect information about their mitotic history. To study the turnover of stem cells in patients with bone marrow failure syndromes, the telomere length in peripheral blood granulocytes and lymphocytes from patients with aplastic anemia (AA, n = 56) and hemolytic paroxysmal nocturnal hemoglobinuria ...

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Progressive telomere shortening in aplastic anemia.

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Aplastic anemia is a fatal bone marrow disorder characterized by peripheral pancytopenia and marrow hypoplasia. The disease can be hereditary or acquired and develops at any stage of life. A subgroup of the inherited form is caused by replicative impairment of hematopoietic stem and progenitor cells due to very short telomeres as a result of mutations in telomerase and other telomere components...

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ژورنال

عنوان ژورنال: International Journal of Molecular Medicine

سال: 2014

ISSN: 1107-3756,1791-244X

DOI: 10.3892/ijmm.2014.1898